Hemoglobin

Preferred Specimen(s) 3 ml Whole blood full EDTA (lavender-top) tube

Instructions Maintain specimen at room temperature. If multiple draw, collect EDTA (lavender-top) tube last. Traumatic tap can introduce thromboplastin and trap WBC and platelets. Refrigeration can precipitate fibrin and trap WBC and platelets. Do not refrigerate.

Transport Container EDTA (lavender-top) tube

Transport Temperature Room temperature

Reject Criteria

  • Hemolysis
  • Clotted
  • Received frozen

Methodology Electronic Cell Sizing, Sorting/Cytometry/Microscopy

Clinical Significance:  Usual method for determining anemia. Used to calculate indices.