Testosterone, Free, Bioavailable and Total, LC/MS/MS

Includes Total Testosterone, Free and Bioavailable Testosterone (calculated), Sex Hormone Binding Globulin, Albumin

Preferred Specimen(s) 2.5 mL serum collected in a (red-top) tube (no gel)

Instructions Specify age and sex on test requisition. Shipping frozen is acceptable.

Transport Container Plastic screw-cap vial

Transport Temperature Refrigerated (cold packs)

Reject Criteria Samples received in serum separator tubes (SST®s)

Methodology

  • Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS)
  • Calculation
  • Spectrophotometry
  • Immunochemiluminescent Assay

Clinical Significance Helpful in assessing testicular function in males and managing hirsutism, virilization in females.