Includes D2, D3 and Total Vitamin D, 25-Hydroxy
Preferred Specimen(s) 1 mL serum
Instructions Collect blood by venipuncture into plain tubes (without anticoagulant). Separate the serum from the cells immediately.
Transport Container Plastic screw-cap vial
Transport Temperature Frozen; 2-8oC
Reject Criteria
- Gross hemolysis
- Gross lipemia
- Plasma
Methodology CLIA
Clinical Significance This assay should be used to monitor patients taking vitamin D supplements as well as to diagnose Vitamin D deficiency. The measurement of 25-OH Vitamin D is the best indicator of Vitamin D status in the body. This assay employs chemiluminescence and measures the total of 25-OH D2 (the analog form used to treat 25-OH D3 deficiency) and 25-OH D3( the endogenous form of the vitamin).